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KMID : 1094720140190050844
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 5 p.844 ~ p.850
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Synthesis of cefprozil using penicillin G acylase in recyclable aqueous two-phase systems
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Zhu Chaohui
Cao Xuejun
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Abstract
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Cefprozil is an important semi-synthetic cephalosporin antibiotic. In this study, immobilized penicillin G acylase (PGA) is used to catalyze the acylation of 7- amino-3-(1-propenyl)-4-cephalosporanic acid (7-APRA) and 4-hydroxyphenylglycine methyl ester (HPGME) and a recyclable thermo-pH responsive PNB/PADB aqueous twophase system (ATPS) is used to synthesize cefprozil. In this system, the partition coefficient of cefprozil was 2.24 with 60 mmol/L (NH4)2SO4. In addition, the optimal enzymatic reaction conditions were found to be pH 6.5, 20°C, 78 u/mL immobilized PGA, 30 mmol/L 7-APRA and 90 mmol/L HPGME. In the PNB/PADB ATPS, the maximal yield of cefprozil was 75.81% with 60 mmol/L (NH4)2SO4 and in the single aqueous system the yield was 56.02%. The yields are thought to improve because there is a reduction in product inhibition.
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KEYWORD
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cefprozil, product inhibition, immobilized penicillin G Acylase, bioconversion, recyclable aqueous two-phase systems
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